Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids

• Oxidation of polyunsaturated fatty acids can yield an array of eicosanoids.
• These lipid mediators play key pathophysiological roles in humans.
• Mass spectrometry-based technology allows us to analyze such lipid mediators.
• Development of high-throughput approaches facilitates the analysis of eicosanoids.

Oxidation of polyunsaturated fatty acids (PUFA) through enzymatic or non-enzymatic free radical-mediated reactions can yield an array of lipid metabolites including eicosanoids, octadecanoids, docosanoids and related species. In mammals, these oxygenated PUFA mediators play prominent roles in the physiological and pathological regulation of many key biological processes in the cardiovascular, renal, reproductive and other systems including their pivotal contribution to inflammation. Mass spectrometry-based technology platforms have revolutionized our ability to analyze the complex mixture of lipid mediators found in biological samples, with increased numbers of metabolites that can be simultaneously quantified from a single sample in few analytical steps. The recent development of high-sensitivity and high-throughput analytical tools for lipid mediators affords a broader view of these oxygenated PUFA species, and facilitates research into their role in health and disease. In this review, we illustrate current analytical approaches for a high-throughput lipidomic analysis of eicosanoids and related mediators in biological samples. This article is part of a Special Issue entitled “Oxygenated metabolism of PUFA: analysis and biological relevance.”

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