کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1950058 | 1537799 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protein kinase CβI interacts with the β1-adrenergic signaling pathway to attenuate lipolysis in rat adipocytes
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کلمات کلیدی
β-ARbuffer Cbuffer Binsulin-receptor substratenPKCconventional PKCPhosphodiesterase 3BNovel PKCcPKCaPKCHSLphorbol 12-myristate 13-acetatePKBPDE3BPKCPI3KSDSHEPESpKaIRS4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid - 4- (2-hydroxyethyl) -1-piperazineethanesulfonic acidBSA - BSAPMA - LDC هاAtypical PKC - PKC آتیپیکAdipocyte - آدیپوسیتbovine serum albumin - آلبومین سرم گاوbuffer A - بافر Adiacylglycerol - دیسیل گلیسیرینDAG - روزsodium dodecylsulphate - سدیم دودسیل سولفاتPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازhormone-sensitive lipase - لیپاز حساس به هورمونLipolysis - لیپولیزprotein kinase A - پروتئین کیناز Aprotein kinase B - پروتئین کیناز BProtein kinase C - پروتئین کیناز سیβ-Adrenergic receptor - گیرنده β-adrenergic
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have shown previously that insulin attenuates β1-adrenergic receptor (β1-AR)-mediated lipolysis via activation of protein kinase C (PKC) in rat adipocytes. This antilipolysis persists after removal of insulin and is independent of the phosphodiesterase 3B activity, and phorbol 12-myristate 13-acetate (PMA) could substitute for insulin to produce the same effect. Here, we attempted to identify the PKC isoform responsible for antilipolysis. Isolated adipocytes were treated with high and low concentrations of PMA for up to 6 h to degrade specific PKC isoforms. In the PMA-treated cells, the downregulation profiles of PKC isoforms α and βI, but not βII, δ, É, or ζ, correlated well with a decrease of lipolysis-attenuating effect of PMA. After rats fasted for 24 h, adipocyte expression of PKC isoform α increased, while expression of PKCδ decreased. Fasting did not change the potency of PMA to attenuate lipolysis, however. The lipolysis-attenuating effect of PMA was blocked by the PKCβI/βII inhibitor LY 333531, but not by the PKCβII inhibitor CGP 53353 or the PKCδ inhibitor rottlerin. These data suggest that PKCβI interacts with β1-AR signaling and attenuates lipolysis in rat adipocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1781, Issue 5, May 2008, Pages 277-281
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1781, Issue 5, May 2008, Pages 277-281
نویسندگان
Jiro Nakamura,