کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
195110 459806 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Signal amplification for impedimetric genosensing using gold-streptavidin nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Signal amplification for impedimetric genosensing using gold-streptavidin nanoparticles
چکیده انگلیسی

Streptavidin-coated gold nanoparticles (strept-AuNPs) were used in this work to amplify the impedimetric signal generated in a biosensor detecting the DNA hybridization event. Probe oligomer was adsorbed onto a graphite epoxy composite (GEC) electrode surface and the impedance measurement was performed in a solution containing the redox marker ferrocyanide/ferricyanide. The biotinylated complementary oligomer was used as target. The change of interfacial charge transfer resistance (Rct), experimented by the redox marker, was recorded to confirm the hybrid formation. The addition of strept-AuNPs, binding to the target due to the strong streptavidin–biotin interaction, led to a further increment of Rct thus obtaining significant signal amplification. Strept-AuNPs on the electrode surface were observed by scanning electron microscopy (SEM) after silver enhancement treatment. A competitive binding assay was also performed using unlabelled DNA target to demonstrate its applicability to real sample analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 53, Issue 11, 20 April 2008, Pages 4022–4029
نویسندگان
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