کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1952170 | 1057182 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antitumour activity on extrinsic apoptotic targets of the triterpenoid maslinic acid in p53-deficient Caco-2 adenocarcinoma cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
FACSRh123CDDOFCSDAPIDMEMMMP4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMaslinic acid - اسید ماسلینیکTriterpènes - تریترپنسDimethyl sulfoxide - دیمتیل سولفواکسیدRhodamine - رودامینfoetal calf serum - سرم گوساله جنینColon cancer cells - سلول های سرطانی کولونfluorescence-activated cell sorter - فلورسانس فعال سلول مرتب سازMitochondrial membrane potential - پتانسیل غشای میتوکندریPropidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We report that a novel triterpenoid, (2a,3b)-2,3-dihydroxyolean-12-en-28-oic acid (maslinic acid), isolated from olive pomace from Olea europaea, triggers primarily the extrinsic and later the intrinsic apoptotic pathways in Caco-2 human colon-cancer cells. Apoptosis induced by maslinic acid was confirmed by FACS analysis using annexine-V FICT staining. This induction of apoptosis was correlated with the early activation of caspase-8 and caspase-3, the activation of caspase-8 was also correlated with higher levels of Bid cleavage and decreased Bcl-2, but with no change in Bax expression. Maslinic acid also induced a sustained activation of c-Jun N-terminal kinase (JNK). Incubation with maslinic acid also resulted in the later activation of caspase-9, which, together with the lack of any Bax activation, suggests that the mitochondrial pathway is not required for apoptosis induced by maslinic acid in this cell line. In this study we found that the mechanism of apoptotic activation in p53-deficient Caco-2 cells differs significantly from that found in HT-29 cells. Natural agents able to activate both the extrinsic and intrinsic apoptotic pathways by avoiding the mitochondrial resistance mechanisms may be useful for treatment against colon cancer regardless of its aetiology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 95, Issue 11, November 2013, Pages 2157-2167
Journal: Biochimie - Volume 95, Issue 11, November 2013, Pages 2157-2167
نویسندگان
Fernando J. Reyes-Zurita, Eva E. Rufino-Palomares, Pedro P. Medina, E. Leticia GarcÃa-Salguero, Juan Peragón, Marta Cascante, José A. Lupiáñez,