Importance of pH and disulfide bridges on the structural and binding properties of human α1-acid glycoprotein

► Reduced AGP is less thermostable than its oxidized counterpart. ► A heat-induced molten globule-like state was detected at pH 7.4, 5.5 and pH 4.5. ► pH and disulfide bridges act as modulators of the MG formation. ► Disulfide bridges are important for the binding and structural properties of AGP.