کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1952994 | 1057243 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HIV-1 integrase-hydrolyzing antibodies from sera of HIV-infected patients
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کلمات کلیدی
VIPABZHIV-1 integrase - HIV-1 انتگرالHIV-1 reverse transcriptase - HIV-1، ترانسپرتایز معکوسAbzyme - ابزیمSDS-polyacrylamide gel electrophoresis - الکتروفورز ژل SDS-polyacrylamide gelSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدHIV-infected patients - بیماران آلوده به ویروس HIVAutoimmune - خودایمنیRelative activity - فعالیت نسبیSystemic lupus erythematosus - لوپوس اریتماتوی سیستمیکSLE - لوپوس منتشر یا لوپوس اریتماتوس سیستمیکAntibody - پادتَن یا آنتیبادی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Autoantibodies with enzymic activities (abzymes) are a distinctive feature of autoimmune diseases. It was interesting whether Abs from patients with viral diseases can hydrolyze viral proteins. Electrophoretically and immunologically homogeneous IgGs were isolated from sera of AIDS patients by chromatography on several affinity sorbents. We present evidence showing that 89.5% IgGs purified from the sera of HIV-infected patients using several affinity resins including Sepharose with immobilized integrase specifically hydrolyze only HIV integrase (IN) but not many other tested proteins. Several rigid criteria have been applied to show that the IN-hydrolyzing activity is an intrinsic property of AIDS IgGs but not from healthy donors. Similar to autoimmune proteolytic abzymes, IN-hydrolyzing IgGs from some patients were inhibited by specific inhibitors of serine and metal-dependent proteases but a significant inhibition of the activity by specific inhibitors of acidic- and thiol-like proteases was observed for the first time. Although HIV infection leads to formation of Abs to many viral and human antigens, no possible biological role for most of them is known. Since anti-IN IgG can efficiently hydrolyze IN, a positive role of abzymes in counteracting the infection cannot be excluded. In addition, detection of IN-hydrolyzing activity can be useful for diagnostic purposes and for estimation of the immune status in AIDS patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimie - Volume 91, Issue 9, September 2009, Pages 1081-1086
Journal: Biochimie - Volume 91, Issue 9, September 2009, Pages 1081-1086
نویسندگان
Svetlana V. Baranova, Valentina N. Buneva, Marina A. Kharitonova, Ludmila P. Sizyakina, Christina Calmels, Marie-Line Andreola, Vincent Parissi, Georgy A. Nevinsky,