Albumin removal from human fibrinogen preparations for manufacturing human fibrin-based biomaterials

• Fibrin is an ideal biomaterial choice for tissue engineering applications.
• Human fibrin sealants allow little room for further tuning of the biomaterial.
• Human fibrinogen solutions offer a cost-effective and versatile alternative.
• However, human fibrinogen solutions contain impurities (albumin and salts).
• We propose a simple method to remove albumin from human fibrinogen solutions.

Commercially available two component human fibrin sealants are commonly used to manufacture human fibrin-based biomaterials. However, this method is costly and allows little room for further tuning of the biomaterial. Human fibrinogen solutions offer a more cost-effective and versatile alternative to manufacture human fibrin-based biomaterials. Yet, human fibrinogen is highly unstable and contains certain impurities like human albumin. Within the context of biomaterials and tissue engineering we offer a simple yet novel solution based on classical biochemical techniques to significantly reduce albumin in human fibrinogen solutions. This method can be used for various tissue engineering and biomedical applications as an initial step in the manufacturing of human fibrin-based biomaterials to optimise their regenerative application.

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