کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
195721 | 459820 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Novel amperometric glucose biosensor based on an ether-linked cobalt(II) phthalocyanine–cobalt(II) tetraphenylporphyrin pentamer as a redox mediator Novel amperometric glucose biosensor based on an ether-linked cobalt(II) phthalocyanine–cobalt(II) tetraphenylporphyrin pentamer as a redox mediator](/preview/png/195721.png)
The development of cobalt(II) phthalocyanine–cobalt(II) tetra(5-phenoxy-10,15,20-triphenylporphyrin), (CoPc–(CoTPP)4) pentamer as a novel redox mediator for amperometric enzyme electrode sensitive to glucose is described. A glassy carbon electrode (GCE) was first modified with the pentamer, then followed by the immobilization onto the GCE–CoPc–(CoTPP)4 with glucose oxidase (GOx) through cross-linking with glutaraldehyde in the presence of bovine serum albumin (BSA) and Nafion® cation-exchange polymer. The proposed biosensor displayed good amperometric respose charateristics to glucose in pH 7.0 PBS solution; such as low overpotentials (+400 mV versus Ag|AgCl), very fast amperometric response time (∼5 s), linear concentration range extended up to 11 mM, with 10 μM detection limit. The biosensor exhibited electrochemical Michaelis–Menten kinetics and showed an average apparent Michaelis–Menten constant (K′M)(K′M) of 14.91 ± 0.46 mM over a storage period of 2 weeks.
Journal: Electrochimica Acta - Volume 51, Issue 24, 15 July 2006, Pages 5131–5136