کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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196050 | 459831 | 2007 | 6 صفحه PDF | دانلود رایگان |

Natural nano-structural attapulgite clay was purified by mechanical stirring with the aid of ultrasonic wave and its structure and morphology was investigated by XRD and transmission electron microscopy (TEM). Cytochrome c was immobilized on attapulgite modified glassy carbon electrode. The interaction between Cytochrome c and attapulgite clay was examined by using UV–vis spectroscopy and electrochemical methods. The direct electron transfer of the immobilized Cytochrome c exhibited a pair of redox peaks with formal potential (E0′)(E0′) of about 17 mV (versus SCE) in 0.1 mol/L, pH 7.0, PBS. The electrode reaction showed a surface-controlled process with the apparent heterogeneous electron transfer rate constant (ks) of 7.05 s−1 and charge-transfer coefficient (α) of 0.49. Cytochrome c immobilized on the attapulgite modified electrode exhibits a remarkable electrocatalytic activity for the reduction of hydrogen peroxide (H2O2). The calculated apparent Michaelis–Menten constant (Kmapp) was 470 μmol/L, indicating a high catalytic activity of Cytochrome c immobilized on attapulgite modified electrode to the reduction of H2O2. Based on these, a third generation of reagentless biosensor can be constructed for the determination of H2O2.
Journal: Electrochimica Acta - Volume 52, Issue 11, 1 March 2007, Pages 3601–3606