کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1967014 1645464 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Therapeutic drug monitoring of topiramate by liquid chromatography-tandem mass spectrometry
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Therapeutic drug monitoring of topiramate by liquid chromatography-tandem mass spectrometry
چکیده انگلیسی

BackgroundTopiramate (TPM) is a new antiepileptic drug (AED) used worldwide in patients with various types of epilepsies and also for prophylaxis of migraine. A rapid, selective, reliable, precise, accurate, and reproducible tandem mass spectrometric (MS/MS) method for quantification of TPM in human plasma using topiramate-d12 as an internal standard (IS) has been developed and validated to be used routinely for TDM of TPM.MethodsThe drug and IS were extracted by ether and analyzed on Symmetry® C18 column. Quantitation was achieved using ESI-interface employing MRM mode.ResultsThe method was validated over the concentration range of 0.5–30 µg/ml (r > 0.99). Intra- and inter-run precisions of TPM assay at three concentrations ranged from 0.7 to 7.8% with accuracy (bias) varied from − 10.0 to 2.1% indicating good precision and accuracy. Analytical recoveries of TPM and IS from spiked human plasma were in the range of 84.1 to 90.0% and 90.0 to 111.0%, respectively. Stability of TPM in human plasma samples at different conditions showed that the drug was stable under the studied conditions. Matrix effect study showed a lack of matrix effect on mass ions of TPM and IS.ConclusionThe described method compared well when assessed by Heathcontrol TDM theme program (r > 0.99). The suitability of the developed method for TDM was demonstrated by measuring TPM in human plasma samples of epileptic patients treated with TPM. The proposed method is appropriate for routine TDM of TPM.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 411, Issues 9–10, 2 May 2010, Pages 729–734
نویسندگان
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