کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1967558 1538746 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of combined DNA-based piezoelectric biosensors for the simultaneous detection and genotyping of high risk Human Papilloma Virus strains
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Development of combined DNA-based piezoelectric biosensors for the simultaneous detection and genotyping of high risk Human Papilloma Virus strains
چکیده انگلیسی

BackgroundHuman Papilloma Virus (HPV) is a DNA virus belonging to the Papovavirus family. Genital HPV types have been subdivided into medium-low risk, and high-risk (HPV 16 and 18), frequently associated with cervical cancer. Three DNA-based piezoelectric biosensors were here developed for a quick detection and genotyping of HPV.MethodsWe developed a method for the detection and genotyping of HPV in human cervical scraping samples based on coupling DNA piezoelectric sensors with Polymerase Chain Reaction (PCR). The novelty of this work was the design and immobilisation of a degenerate probe (chosen in a conserved region of the viral genome) for the simultaneous detection of 16 virus strains and of two specific probes (chosen in a less-conserved region of the viral genome) for genotyping.ResultsThe three biosensors were optimised with synthetic oligonucleotides with good reproducibility (HPVdeg CV% av 9%, HPV16 CV%av 9%; HPV18 CV%av 11%) with a detection limit of 50 nM. Cervical scraping samples after PCR amplification (in 40–200 nM range), were tested without the need of label with high selectivity and reproducibility. The results were in agreement with a reference method used in routinary analysis.ConclusionPiezoelectric biosensors have proven to be suitable for detection and genotyping of HPV.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 383, Issues 1–2, August 2007, Pages 140–146
نویسندگان
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