کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1967659 1538750 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Establishment of a sandwich ELISA using commercial antibody for plasma or serum 3-nitrotyrosine (3NT). Elevation in inflammatory diseases and complementary between 3NT and myeloperoxidase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Establishment of a sandwich ELISA using commercial antibody for plasma or serum 3-nitrotyrosine (3NT). Elevation in inflammatory diseases and complementary between 3NT and myeloperoxidase
چکیده انگلیسی

BackgroundAmino acid tyrosine residue of a protein can be nitrated to form 3-nitrotyrosine (3NT), which is now being considered as a marker of inflammation, oxidative and nitrosative stress.MethodAn in-house ELISA has been established using the same commercial antibody for both binding and detection of 3NT containing proteins.ResultsThe sensitivity of the in-house ELISA was 1.8 nmol/l. The imprecision was < 10% at all concentrations. The in-house assay correlates well with a commercial kit (r = 0.89). In addition to EDTA plasma, we found that both heparinized plasma and serum can also be used to quantify 3NT concentration. Using the in-house ELISA we have detected increased concentrations of 3NT in diseases known to be associated with inflammation and also in subjects with polyps. As marker of oxidative stress and inflammation, both 3NT and myeloperoxidase are complementary to each other in test sensitivity.ConclusionThis ELISA can be used in the clinical laboratories to monitor the inflammatory disease activity and assess early risks that are associated with inflammation, oxidative and nitrosative stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 378, Issues 1–2, March 2007, Pages 175–180
نویسندگان
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