کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1967714 1538751 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083: Its pathophysiological changes in human gastric juice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Quantitative determination of gland mucous cells-type mucin using a monoclonal antibody, HIK1083: Its pathophysiological changes in human gastric juice
چکیده انگلیسی

BackgroundPathological alteration in gastric mucosa is caused by Helicobacter pylori infection and is detectable by histological analysis. In particular, the alteration of gland mucous cells (GMCs)-type mucin, which plays a protective role against H. pylori infection, is critical in the pathogenesis of H. pylori-related gastritis. We established an assay for GMCs-type mucin and quantitatively assessed the pathophysiological changes in its content in human gastric juice samples.MethodsThe assay method for GMCs-type mucin was based on ELISA using a monoclonal antibody (HIK1083), and was used it to measure GMCs-type mucin in gastric juice obtained from patients with or without H. pylori infection.ResultsAll the basic characteristics of the current method were satisfactory to quantify the GMCs-type mucin content in gastric juice. The GMCs-type mucin content, but not total mucin content, was significantly higher in patients with H. pylori infection (n = 17; 437 ± 476 U, mean ± SD) than in those without H. pylori infection (n = 55; 168 ± 322 U, p < 0.05).ConclusionsThe current method is suitable for the quantitative analysis of GMCs-type mucin in gastric juice. The change in GMCs-type mucin content in gastric juice may be possibly implicated in the pathophysiology of the gastric mucosa and in the patient's gastric mucosal lesions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 377, Issues 1–2, 2 February 2007, Pages 261–267
نویسندگان
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