کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1968028 1538758 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and cross-validation of sequencing-based assays for genotyping common polymorphisms of the CXCL5 gene
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Development and cross-validation of sequencing-based assays for genotyping common polymorphisms of the CXCL5 gene
چکیده انگلیسی

BackgroundEpithelial neutrophil activating peptide (ENA-78) is encoded by the polymorphic CXCL5 gene and is a recruiter and activator of neutrophils. Furthermore, ENA-78 may be involved in pathological inflammatory processes and variable drug responses.MethodsTo facilitate future disease–gene and pharmacogenetic investigation of ENA-78, we developed and cross-validated medium- to high-throughput genotyping assays for 2 commonly occurring CXCL5 polymorphisms (rs352046 and rs425535). Furthermore, we compared allele and genotype frequencies in a U.S. population with those of a previously studied European population.ResultsThere was 100% genotype concordance between the 2 methods used (Pyrosequencing® and TaqMan®). Variant allele frequencies for rs352046 were consistent between the U.S. (16%) and European (16%) populations, while the rs425535 variant allele was more than twice as high in the European cohort (38% vs. 16%). There was complete linkage of genotypes at both loci in our population.ConclusionsThe distribution of variant alleles for the 2 polymorphisms studied should be further evaluated in other populations. In addition, our data highlight the importance of assay validation using multiple platforms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Clinica Chimica Acta - Volume 370, Issues 1–2, August 2006, Pages 72–75
نویسندگان
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