کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1975332 | 1060617 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene expression and activity of digestive enzymes during the larval development of Asian seabass (Lates calcarifer)
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کلمات کلیدی
α-tubLAPDPHBALCTRUBQBile salt activated lipaseFish larvaEF-1αGAPDHAsian seabass - seabass آسیاییα-Tubulin - α-توبولینβ-actin - β-اکتینALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییamylase - آمیلازLeucine aminopeptidase - آمینوپپتیداز لوسینAMY - امیOntogeny - انتگرالGene expression - بیان ژنtrypsinogen - تریپسینوژنTRY - تلاش کردنdays post hatch - روزه گرفتنelongation factor-1α - ضریب انقباض 1αActb - عملpepsinogen - پپسینوژنquantification cycle - چرخه محاسبهChymotrypsinogen - چیوتریپسینوژنglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازDigestion - گوارش یا هضم یا تحلیلUbiquitin - یوبیکویتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Gene expression and activity of digestive enzymes during the larval development of Asian seabass (Lates calcarifer) Gene expression and activity of digestive enzymes during the larval development of Asian seabass (Lates calcarifer)](/preview/png/1975332.png)
چکیده انگلیسی
Knowledge of digestive enzyme development during larval stages provides a better understanding of the digestive and nutritional physiology of fish larvae. This study characterized the ontogeny of key digestive enzymes in Asian seabass larvae from hatching to juvenile stage (30 days post hatch, dph) using molecular and biochemical approaches. Gene expression and activity of pepsinogen (pg), trypsinogen (try), chymotrypsinogen (ctr), bile salt-activated lipase (bal), α-amylase (amy), leucine aminopeptidase (lap) and alkaline phosphatase (alp) were determined. Gene expression and enzyme activity of all digestive enzymes were detectable from hatching. Pepsinogen mRNA levels were close to detection limit during 0-15 dph, but were highly expressed from 18 dph and onwards. This coincided with a marked increase in specific and individual pepsin activity, indicating complete development of digestive function. Expression levels of try, ctr, amy and bal were high between 3 and 15 dph and thereafter a decreasing trend was observed. Intestinal enzymes, lap and alp, showed highest expression levels during the yolk sac stage, and thereafter levels decreased. Activity of all digestive enzymes increased from around 18 dph and onwards. In conclusion, the development of main digestive enzymes in Asian seabass larvae shows a similar pattern to that of other marine fish species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 165, Issue 1, May 2013, Pages 1-9
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 165, Issue 1, May 2013, Pages 1-9
نویسندگان
Manee Srichanun, Chutima Tantikitti, Prapaporn Utarabhand, Trond M. Kortner,