Molecular cloning and characterization of KIFC1-like kinesin gene (ot-kifc1) from Octopus tankahkeei

Spermiogenesis in Octopus tankahkeei involves striking cellular reorganization to generate a mature spermatozoon. This process may require spermatid-specific adaptation of cytoskeleton and associated molecular motor proteins. KIFC1 is a C-terminal kinesin motor with important roles in acrosome biogenesis and nuclear reshaping during spermiogenesis in rat. Here, we have cloned and characterized the gene encoding a homologue of rat KIFC1, termed as ot-kifc1, from the testis of O. tankahkeei. The 2229 bp complete cDNA contains a 75 bp 5′-untranslated region, a 1992 bp open reading frame and a 162 bp 3′-untranslated region. The deduced protein shares an overall identity of 40%, 41%, 39% and 41% with its counterpart from human, rat, mouse and African clawed frog, respectively. Tissue expression analysis revealed ot-kifc1 was expressed in testis, gill and hepatopancreas, but not in other tissues examined. In situ hybridization result showed the ot-kifc1 message was hardly detectable in early spermatid, concentrated at the tail region of intermediate spermatid, abundant in spermatid undergoing dramatic elongation and compression, enriched at one end in late spermatids and disappeared in mature sperm. In conclusion, the expression of ot-kifc1 at specific stages of spermiogenesis suggests a role for this motor in major cytological transformations.