کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1976116 | 1060676 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and biochemical characterization of cytosolic glutathione-S-transferase from filarial worms Setaria cervi
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کلمات کلیدی
Setaria cervi - Setaria CerviHelminth parasites - انگل HelminthCharacterization - تعیین مشخصاتFilariasis - فیلاریایسGlutathione metabolism - متابولیسم گلوتاتیونEnzyme inhibition - مهار آنزیمDrug target - هدف مواد مخدرPurification - پاکسازیglutathione-S-transferase - گلوتاتیون S-ترانسفرازglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study reports the purification and characterization of GST from cytosolic fraction of Setaria cervi. GST activity was determined in various subcellular fractions of bovine filarial worms S. cervi (Bubalus bubalis Linn.) and was found to be localized mainly in the cytosolic and microsomal fractions. The soluble enzyme from S. cervi was purified to homogeneity using a combination of salt precipitation, centrifugation, cation exchange and GSH-Sepharose affinity chromatography followed by ultrafiltration. SDS-PAGE analysis revealed a single band and activity staining was also detected on PAGE gels. Gel filtration and MALDI-TOF studies revealed that the native enzyme is a homodimer with a subunit molecular mass of 24.6Â kDa. Comparison of kinetic properties of the parasitic and mammalian enzymes revealed significant differences between them. The substrate specificity and inhibitor profile of cytosolic GST from S. cervi appeared to be different from GST from mammalian sources.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 151, Issue 3, November 2008, Pages 237-245
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 151, Issue 3, November 2008, Pages 237-245
نویسندگان
Rumana Ahmad, Arvind K. Srivastava, Rolf D. Walter,