Cytochrome P450-dependent monooxygenase system mediated hydrocarbon metabolism and antioxidant enzyme responses in prawn, Macrobrachium malcolmsonii

We investigated the alteration of cytochrome P450-dependent monooxygenase enzymes and antioxidant enzymes in response to oil effluent in freshwater prawn, Macrobrachium malcolmsonii. The prawns were exposed to two sublethal (10% [0.91 ppt] and 25% [2.3 ppt] of 5-day median lethal concentration) concentrations of oil. After 30 days, treated prawns were transferred into untreated freshwater and depuration was followed for another 30 days. At 7-day intervals, hydrocarbons and detoxifying enzymes were analysed in the hepatopancreas. Accumulation of hydrocarbon in the tissues gradually increased when exposed to sublethal concentration of oil effluent associated with enhanced levels of cytochrome P450, NADPH cytochrome c reductase and cytochrome b5. During depuration, the levels of accumulated hydrocarbons decreased due to the induction of these detoxifying enzymes. Oil derived hydrocarbon mediated oxyradical production would have occurred in M. malcolmsonii. This was confirmed by elevated levels of superoxide dismutase (SOD) and catalase (CAT). Thus, cytochrome P450-dependent monooxygenase enzymes and antioxidant enzymes in oil-exposed prawns demonstrate a well-established detoxifying mechanism in M. malcolmsonii.