کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1978808 | 1061625 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular evolution of nitric oxide synthases in metazoans
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کلمات کلیدی
flavin adenine dinucleotideFMNNADPHPDZiNOSeNOSnNOSendothelial NOS - اندوتلیال NOSFAD - بدMolecular evolution - تکامل مولکولیCAM - ساخت به کمک کامپیوترinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییendothelial nitric oxide synthase - سنتاز اکسید نیتریک اندوتلیالneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیNitric oxide synthases - سنتز اکسید نیتروژنflavin mononucleotide - فلاون مونونوکلئوتیدnicotinamide-adenine dinucleotide phosphate - نیکوتین آمید آدنین دیونوکلئوتید فسفاتCalmodulin - کالمودولین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nitric oxide synthases (NOS), the enzymes responsible for the NO synthesis, are present in all eukaryotes. Three isoforms (neuronal, inducible and endothelial), encoded by different loci, have been described in vertebrates, although the endothelial isoform seems to be restricted to tetrapods. In invertebrates, a variety of NOS isoforms have been variably annotated as “inducible” or “neuronal”, while others lack precise annotation. We have performed an exhaustive collection of the available NOS amino-acid sequences in order to perform a phylogenetic analysis. We hypothesized that the NOS isoforms reported in vertebrates derive from 1) different invertebrate NOS, 2) a single invertebrate ancestral gene, through an event related to the double whole genomic duplication that occurred at the origin of vertebrates, and 3) the endothelial form of NOS appeared late in the evolution of vertebrates, after the split of tetrapods and fishes. Our molecular evolution analysis strongly supports the second scenario, the three vertebrate NOS isoforms derived from a single ancestral invertebrate gene. Thus, the diverse NOS isoforms in invertebrates can be explained by events of gene duplication, but their characterization as “inducible” or “neuronal” should only be justified by physiological features, since they are evolutionarily unrelated to the homonym isoforms of vertebrates.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 5, Issue 4, December 2010, Pages 295-301
Journal: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Volume 5, Issue 4, December 2010, Pages 295-301
نویسندگان
C.M. González-Domenech, R. Muñoz-Chápuli,