کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1979962 1539385 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SNF2H interacts with XRCC1 and is involved in repair of H2O2-induced DNA damage
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
SNF2H interacts with XRCC1 and is involved in repair of H2O2-induced DNA damage
چکیده انگلیسی


• CK2-dependent phosphorylation of XRCC1 is crucial for its interaction with SNF2H.
• Foci-formation of XRCC1 and PAR in response to H2O2 treatment is dependent on SNF2H.
• Knock-down of SNF2H impede repair of H2O2-induced lesion.

The protein XRCC1 has no inherent enzymatic activity, and is believed to function in base excision repair as a dedicated scaffold component that coordinates other DNA repair factors. Repair foci clearly represent the recruitment and accumulation of DNA repair factors at sites of damage; however, uncertainties remain regarding their organization in the context of nuclear architecture and their biological significance. Here we identified the chromatin remodeling factor SNF2H/SMARCA5 as a novel binding partner of XRCC1, with their interaction dependent on the casein kinase 2-mediated constitutive phosphorylation of XRCC1. The proficiency of repairing H2O2-induced damage was strongly impaired by SNF2H knock-down, and similar impairment was observed with knock-down of both XRCC1 and SNF2H simultaneously, suggesting their role in a common repair pathway. Most SNF2H exists in the nuclear matrix fraction, forming salt extraction-resistant foci-like structures in unchallenged nuclei. Remarkably, damage-induced formation of both PAR and XRCC1 foci depended on SNF2H, and the PAR and XRCC1 foci co-localized with the SNF2H foci. We propose a model in which a base excision repair complex containing damaged chromatin is recruited to specific locations in the nuclear matrix for repair, with this recruitment mediated by XRCC1-SNF2H interaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 43, July 2016, Pages 69–77
نویسندگان
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