کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1981199 | 1061906 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Poly(ADP-ribose) polymerase-1 modulates DNA repair capacity and prevents formation of DNA double strand breaks
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کلمات کلیدی
DSBPARP-1BERSSBXRCC1DNA polymerase β - DNA پلیمراز بSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNADNA repair - ترمیم DNAbase excision repair - تعمیر پایه پایهdouble strand break - شکست دو رشتهsingle strand break - شکستن تک رشتهpol β - پل بPoly(ADP-ribose) polymerase-1 - پلی (ADP-ribose) پلیمراز-1
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although poly(ADP-ribose) polymerase-1 (PARP-1) has no enzymatic activity involved in DNA damage processing by the base excision repair (BER) pathway, PARP-1 deficient cells are genetically unstable and sensitive to DNA-damaging agents. To explain this paradox, we investigated the impact of PARP-1 on BER in mammalian cells. We reduced cellular PARP-1 protein levels using siRNA, then introduced DNA damage by hydrogen peroxide treatment and examined the repair response. We find that PARP-1 is not involved in recruitment of the major BER proteins to sites of DNA damage. However, we find that PARP-1 protects excessive DNA single strand breaks (SSBs) from converting into DNA double strand breaks (DSBs) thus preserving them for subsequent repair by BER enzymes. This suggests that PARP-1 plays an important role in BER by extending the ability of BER enzymes to process DNA single strand breaks arising directly after mutagen stress or during processing of DNA lesions following extensive DNA damage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 7, Issue 6, 1 June 2008, Pages 932-940
Journal: DNA Repair - Volume 7, Issue 6, 1 June 2008, Pages 932-940
نویسندگان
Bethany C. Woodhouse, Irina I. Dianova, Jason L. Parsons, Grigory L. Dianov,