کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1982362 | 1062281 | 2011 | 7 صفحه PDF | دانلود رایگان |
Complementary DNAs encoding homologs of the tumor suppressor gene, p53, were characterized from two lepidopteran insects, Bombyx mori (Bm) and Spodoptera frugiperda (Sf). They encoded predicted proteins of 368 (41.2 kDa) (Bm) and 374 (42.5 kDa) (Sf) amino acids. The sequences shared 44% amino acid and 60% nucleotide sequence identity with each other, but exhibited less than 20% amino acid and 46% nucleotide sequence identity to Drosophila melanogaster p53. Despite the sequence diversity, conserved amino acids involved in DNA and zinc binding were present in the lepidopteran sequences. Expression of Sfp53-induced apoptosis in S. frugiperda cells, and antiserum made against recombinant Sfp53 recognized a protein whose abundance increased after treatment with DNA damaging agents.
Figure optionsDownload high-quality image (90 K)Download as PowerPoint slideHighlights
► Spodoptera frugiperda and Bombyx mori p53s show 44% amino acid identity, but only 20% amino acid identity to Drosophila melanogaster p53.
► RACE analysis of B.mori p53 demonstrated 9 exons and 8 exons covering about 10,000 base pairs of chromosome 16.
► S.frugiperda p53 induced apoptosis in Sf9 cells and its expression was elevated in cells treated with DNA damaging agents.
Journal: Insect Biochemistry and Molecular Biology - Volume 41, Issue 8, August 2011, Pages 613–619