Permeabilization of Drosophila embryos for introduction of small molecules

► Natural plant-based solvent removes wax layer of Drosophila eggshell. ► Permeabilized embryos are viable and can take up small molecules. ► Both early and late developmental endpoints can be used as readouts of small molecule activity on development. ► Fluorescent dye facilitates identification of permeabilized viable embryos.