کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1983624 | 1539908 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interactions of the ubiquitous octamer-binding transcription factor-1 with both the signal transducer and activator of transcription 5 and the glucocorticoid receptor mediate prolactin and glucocorticoid-induced β-casein gene expression in mammary epithe
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کلمات کلیدی
EGFPRLRSTAT5snRNAPMSFGREqRT-PCRGAPDHDTTTBPqPCRMECs - MECSQuantitative PCR - PCR کمیsmall nuclear RNA - RNA هسته ای کوچکTATA box-binding protein - TATA جعبه اتصال پروتئینPOU - TOelectrophoresis mobility shift assay - آزمایش تحرک تحرک الکتروفورزOct-1 - اکتبر 1Gene expression - بیان ژنImmunoprecipitation - تخریب ایمنیProtein–protein interactions - تعاملات پروتئین-پروتئینTranscriptional regulation - تنظیم ترانزیتیHormonal regulation - تنظیم هورمونیdithiothreitol - دیتیوتریتولquantitative reverse transcription PCR - رونویسی معکوس PCR کمیMammary epithelial cells - سلول های اپیتلیال پستانEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکepidermal growth factor - عامل رشد اپیدرمیglucocorticoid response elements - عناصر پاسخ گلوکوکورتیکوئیدsignal transducer and activator of transcription 5 - فرستنده سیگنال و فعال کننده رونویسی 5phenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدwild-type - نوع وحشیMilk protein - پروتئین شیرglyceraldehydes-3-phosphate dehydrogenase - گلیسرالید هیدروژن 3-فسفات دهیدروژنازprolactin receptor - گیرنده پرولاکتینglucocorticoid receptor - گیرنده گلوکوکورتیکوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Regulation of milk protein gene expression by lactogenic hormones (prolactin and glucocorticoids) provides an attractive model for studying the mechanisms by which protein and steroid hormones synergistically regulate gene expression. β-Casein is one of the major milk proteins and its expression in mammary epithelial cells is stimulated by lactogenic hormones. The signal transducer and activator of transcription 5 and glucocorticoid receptor are essential downstream mediators of prolactin and glucocorticoid signaling, respectively. Previous studies have shown that mutating the octamer-binding site of the β-casein gene proximal promoter dramatically reduces the hormonal induction of the promoter activity. However, little is known about the underlying molecular mechanisms. In this report, we show that lactogenic hormones rapidly induce the binding of octamer-binding transcription factor-1 to the β-casein promoter and this induction is not mediated by either increasing the expression of octamer-binding transcription factor-1 or inducing its translocation to the nucleus. Rather, lactogenic hormones induce physical interactions between the octamer-binding transcription factor-1, signal transducer and activator of transcription 5, and glucocorticoid receptor to form a ternary complex, and these interactions enhance or stabilize the binding of these transcription factors to the promoter. Abolishing these interactions significantly reduces the hormonal induction of β-casein gene transcription. Thus, our study indicates that octamer-binding transcription factor-1 may serve as a master regulator that facilitates the DNA binding of both signal transducer and activator of transcription 5 and glucocorticoid receptor in hormone-induced β-casein expression, and defines a novel mechanism of regulation of tissue-specific gene expression by the ubiquitous octamer-binding transcription factor-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 3, March 2013, Pages 724-735
Journal: The International Journal of Biochemistry & Cell Biology - Volume 45, Issue 3, March 2013, Pages 724-735
نویسندگان
Xi Qian, Feng-Qi Zhao,