کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1985278 | 1539974 | 2007 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Involvement of the ERK/MAP kinase signalling pathway in milli-calpain activation and myogenic cell migration
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کلمات کلیدی
BCAMEF-2ERK/MAPK pathwayPKBdl-dithiothreitolMARCKSIGF-1ERKFAKTBSEGTADTTPVDFPI3KEGFPBSDMEMFBSTGF-β1DMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoMAPK - MAPKMAPK/ERK kinase - MAPK / ERK kinaseNBT/BCIP - NBT / BCIPethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیست (2-aminoethylether) -N، N، N '، N'-tetraacetic acidinsulin-like growth factor-1 - انسولین مانند عامل رشد 1Tris buffer saline - بیس بال Trisbicinchoninic acid - بیسینکنینیک اسیدTransforming growth factor-β1 - تبدیل فاکتور رشد β1Dimethyl sulfoxide - دیمتیل سولفواکسیدfoetal bovine serum - سرم جنین گاوextracellular-signal regulated kinase - سیگنال تنظیم شده سیگنال خارج سلولیmyocyte enhancer factor-2 - عامل افزایش دهنده myocyte-2epidermal growth factor - عامل رشد اپیدرمیGrowth factors - فاکتور رشدphosphate buffer saline - فسفات بافر شورphosphatidylinositol 3 kinase - فسفاتیدیلینوزیتول 3 کینازMEK - مجاهدین خلقprotein kinase B - پروتئین کیناز Bmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenPolyvinylidene fluoride - پلی وینیلیدین فلورایدfocal adhesion kinase - کیناز چسبندگی کانونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Involvement of the ERK/MAP kinase signalling pathway in milli-calpain activation and myogenic cell migration Involvement of the ERK/MAP kinase signalling pathway in milli-calpain activation and myogenic cell migration](/preview/png/1985278.png)
چکیده انگلیسی
Recent research carried out in our laboratory has shown that IGF-1, TGF-β1, and insulin were able to strongly stimulate myoblast migration by increasing milli-calpain expression and activity. However, the signalling pathways involved in these phenomena remain unknown. The aim of this study was to identify the signalling pathway(s) responsible for the effects of IGF-1, TGF-β1, and insulin on myoblast migration and on milli-calpain expression and activity. For this purpose, wound healing assays were carried out in the presence of growth factors with or without specific inhibitors of ERK/MAP kinase and PI3K/Akt pathways. The results clearly showed that the inhibition of the ERK/MAP kinase pathway prevents the effects of growth factors on myoblast migration. Secondly, the expression and the activity of milli-calpain were studied in cells treated with growth factor, alone or with ERK/MAP kinase inhibitor. The results demonstrated that the up-regulation of milli-calpain expression and activity was mediated by the ERK/MAP kinase pathway. Finally, the possible implication of MyoD and myogenin, myogenic regulatory factors able to regulate milli-calpain expression, was studied. Taken together our results clearly showed that the ERK/MAP kinase signalling pathway is responsible for the effects of the three growth factors on myoblast migration and on milli-calpain expression and activity. On the opposite, the PI3K/Akt signalling pathway, MyoD and myogenin seem to be not implicated in these phenomena.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 39, Issue 6, 2007, Pages 1177-1189
Journal: The International Journal of Biochemistry & Cell Biology - Volume 39, Issue 6, 2007, Pages 1177-1189
نویسندگان
Ludovic Leloup, Laetitia Daury, Germain Mazères, Patrick Cottin, Jean-Jacques Brustis,