کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1986045 | 1540237 | 2016 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of correlation between in-silico and in-vitro test analysis against Leishmania HGPRT to inhibitors
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کلمات کلیدی
GMPFlexXXPRTDGDPKDLHGPRTHprtAPRTMCLPDBRMSDGCM3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideguanosine 3′,5′-cyclic monophosphate - guanosine 3 '، 5'-cyclic monophosphateMTT - MTTBLAST, basic local alignment search tool - ابزار پایهای برای جستجوی برهم نهیهای موضعی، بلاستBlast - انفجارQSAR - بزرگسال Docking - داکتCutaneous leishmaniasis - سالک، لیشمانیوز جلدی، لیشمانیوز پوستیLeishmania - لیشمانیا Visceral leishmaniasis - لیشمانیاز احشایی، کالاآزارpost-kala-azar dermal leishmaniasis - لیشمانیوز پوست پوستی قزلآ آزارroot mean square deviation - میانگین انحراف مربع ریشهhypoxanthine phosphoribosyltransferase - هیپوکسانتین فسفریبوسیل ترانسفرازhypoxanthine-guanine phosphoribosyltransferase - هیپوکسانتین-گوانین فسفریبوسیل ترانسفرازProtein Data Bank - پروتئین بانک اطلاعاتیGuanosine monophosphate - گوئنوسین مونوفسفره
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hypoxanthine Phosphoribosyltransferase (HGPRT; EC 2.4.2.8) is a central enzyme in the purine recycling pathway of all protozoan parasites. Protozoan parasites cannot synthesize purine bases (DNA/RNA) which is essential for survival as lack of de-novo pathway. Thus its good target for drug design and discovery as inhibition leads to cessation of replication. PRTase (transferase enzyme) has common PRTase type I folding pattern domain for its activities. Genomic studies revealed the sequence pattern and identified highly conserved residues that catalyzed the reaction in protozoan parasites. A recombinant protein has 24 kDa molecular mass (rLdHGPRT) was cloned, expressed and purified for testing of guanosine monophosphate (GMP) analogous compounds in-vitro by spectroscopically to the rLdHGPRT, lysates protein and MTT assay on Leishmania donovani. The predicted inhibitors of different libraries were screen into FlexX. The reported inhibitors were tested in-vitro. The 2â²-deoxyguanosine 5â²-diphosphate (DGD) (IC50 value 12.5 μM) is two times more effective when compared to guanosine-5â²-diphosphate sodium (GD). Interestingly, LdHGPRT complex has shown stable after 24 ns in molecular dynamics simulation with interacting amino acids are Glu125, Ile127, Lys87 and Val186. QSAR studies revealed the correlation between predicted and experimental values has shown R2 0.998. Concludes that inversely proportional to their docked score with activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 83, February 2016, Pages 78-96
Journal: International Journal of Biological Macromolecules - Volume 83, February 2016, Pages 78-96
نویسندگان
Md Yousuf Ansari, Asif Equbal, Manas Ranjan Dikhit, Rani Mansuri, Sindhuprava Rana, Vahab Ali, Ganesh Chandra Sahoo, Pradeep Das,