کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1990318 | 1540683 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
4-O-methylhonokiol inhibits colon tumor growth via p21-mediated suppression of NF-κB activity
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کلمات کلیدی
NF-κBDAPIP21c-IAP14-O-methylhonokiol4,6-diamino-2-phenylindole - 4،6-دیامینو-2-فنیلینولelectromobility shift assay - آزمایش الکتریکی حرکتیTUNEL - تونلCancer cells - سلول های سرطانیEMSA یا electrophoretic mobility shift assay - سنجش تغییر تحرک الکتروفورتیکnuclear factor-κB - فاکتور هسته ای κBgrowth inhibition - مهار رشد
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Biphenolic components in the Magnolia family have shown several pharmacological activities such as antitumor effects. This study investigated the effects of 4-O-methylhonokiol (MH), a constituent of Magnolia officinalis, on human colon cancer cell growth and its action mechanism. 4-O-methylhonokiol (0-30 μM) decreased constitutive activated nuclear factor (NF)-κB DNA binding activity and inhibited growth of human colon (SW620 and HCT116) cancer cells. It also caused G0-G1 phase cell cycle arrest followed by an induction of apoptotic cell death. However, knockdown with small interfering RNA (siRNA) of p21 or transfection with cyclin D1/Cdk4 binding site-mutated p21 abrogated MH-induced cell growth inhibition, inhibition of NF-κB activity as well as expression of cyclin D1 and Cdk4. Conversely, inhibition of NF-κB with specific inhibitor or siRNA augmented MH-induced apoptotic cell death. 4-O-methylhonokiol inhibited tumor growth, NF-κB activity and expression of antiapoptotic proteins; however, it increased the expression of apoptotic proteins as well as p21 in xenograft nude mice bearing SW620 cancer cells. The present study reveals that MH causes p21-mediated human colon cancer cell growth inhibition through suppression of NF-κB and indicates that this compound by itself or in combination with other anticancer agents could be useful for the treatment of cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 23, Issue 7, July 2012, Pages 706-715
Journal: The Journal of Nutritional Biochemistry - Volume 23, Issue 7, July 2012, Pages 706-715
نویسندگان
Ju Hoon Oh, Jung Ok Ban, Min-Chul Cho, Miran Jo, Jae Kyung Jung, Byeongwoo Ahn, Do-Young Yoon, Sang Bae Han, Jin Tae Hong,