کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1990924 | 1540758 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of the type of dietary fat on acetylcholine-evoked amylase secretion and calcium mobilization in isolated rat pancreatic acinar cells
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کلمات کلیدی
IP3Cholecystokinin-octapeptidesarcoplasmic/endoplasmic reticulum calcium ATPaseCCK-8PMCAPSSBSA - BSAinositol 1,4,5-trisphosphate - inositol 1،4،5-trisphosphatebovine serum albumin - آلبومین سرم گاوACh - آهAcetylcholine - استیل کولینMembrane fatty acids - اسیدهای چرب غشاءOlive oil - روغن زیتونDietary fat - رژیم چاقیPancreatic acinar cells - سلول های آکینار پانکراسplasma membrane calcium ATPase - غشای پلاسما کلسیم ATPaseSERCA - قلبlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH physiological salt solution - محلول نمک فیزیولوژیکیRat - موش صحراییCell calcium - کلسیم سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of the type of dietary fat on acetylcholine-evoked amylase secretion and calcium mobilization in isolated rat pancreatic acinar cells Effects of the type of dietary fat on acetylcholine-evoked amylase secretion and calcium mobilization in isolated rat pancreatic acinar cells](/preview/png/1990924.png)
چکیده انگلیسی
Olive oil is a major component of the Mediterranean diet, and its role in human health is being actively debated. This study aimed to clarify the mechanism of pancreatic adaptation to dietary fat. For this purpose, we examined whether dietary-induced modification of pancreatic membranes affects acinar cell function in response to the secretagogue acetylcholine (ACh). Weaning male Wistar rats were assigned to one of two experimental groups and fed for 8 weeks with a commercial chow (C) or a semisynthetic diet containing virgin olive oil as dietary fat (OO). The fatty acid composition of pancreatic plasma membranes was determined by gas-liquid chromatography. For assessment of secretory function, viable acini were incubated with ACh and amylase of supernatant was further assayed with a substrate reagent. Changes in cytosolic Ca2+ concentration in response to ACh were measured by fura-2 AM fluorimetry. Compared to C rats, pancreatic cell membranes of OO rats had a higher level of monounsaturated fatty acids and a lower level of both saturated and polyunsaturated fatty acids, thus, reflecting the type of dietary fat given. Net amylase secretion in response to ACh was greatly enhanced after OO feeding, although this was not paralleled by enhancement of ACh-evoked Ca2+ peak increases. In conclusion, chronic intake of diets that differ in the fat type influences not only the fatty acid composition of rat pancreatic membranes but also the responsiveness of acinar cells to ACh. This mechanism may be, at least in part, responsible for the adaptation of the exocrine pancreas to the type of fat available.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 17, Issue 4, April 2006, Pages 242-249
Journal: The Journal of Nutritional Biochemistry - Volume 17, Issue 4, April 2006, Pages 242-249
نویسندگان
MarÃa D. Yago, Ricardo J. DÃaz, MarÃa A. MartÃnez, Nama'a Audi, José A. Naranjo, Emilio MartÃnez-Victoria, Mariano Mañas,