کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1995278 1064960 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein kinase C iota mediates lipid-induced apoptosis of human coronary artery endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Protein kinase C iota mediates lipid-induced apoptosis of human coronary artery endothelial cells
چکیده انگلیسی

Apoptosis is involved in the development and progression of atherosclerotic lesions. Protein kinase C (PKC) signalling is of importance in atherosclerosis as well as apoptosis. Therefore, we tested the involvement of PKC in lipid-induced apoptosis of human coronary artery endothelial cells (HCAEC).Protein expression of PKC isoforms α, βI, δ, ɛ, and ι was detected, whereas no relevant protein amounts of PKC isoforms βII, γ, η, θ, and ζ were found. Inhibition of classical and novel PKC isoforms by treatment with bisindolylmaleimide or PKC down-regulation by long-term treatment with 12-O-tetradecanoyl phorbol-13-acetate (TPA) could not prevent apoptosis induced by palmitate or stearate. In contrast, a specific myristoylated, cell-permeable PKC ζ/ι pseudosubstrate prevented lipid-induced apoptosis in HCAEC. Furthermore, saturated fatty acids activated PKC ι as evidenced by PKC ι down-regulation upon long-term treatment with stearate.Our data provide evidence that PKC ι is activated by saturated fatty acids and mediates lipid-induced apoptosis of HCAEC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microvascular Research - Volume 78, Issue 1, June 2009, Pages 40–44
نویسندگان
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