کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2015177 | 1541973 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and partial purification of acyl-CoA:glycerol 3-phosphate acyltransferase from sunflower (Helianthus annuus L.) developing seeds
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کلمات کلیدی
GPATLPALPAATHelianthus annuusDAFDAGATAcyltransferaseTLCBSA - BSAbovine serum albumin - آلبومین سرم گاوphosphatidic acid - اسید فسفاتیدیکlysophosphatidic acid - اسید لیسفسفیدیدtriacylglycerol - تری آسیل گلیسرول TAG یا triacylglycerols - تری گلیسرید یا تری آسیل گلیسرولSeeds - دانهdiacylglycerol - دیسیل گلیسیرینDiacylglycerol acyltransferase - دیسیللیسرول آکیلتانسفرازازDAG - روزdays after flowering - روز بعد از گلMicrosomal membrane - غشای میکروسیمالCHAPS - چاپسthin-layer chromatography - کروماتوگرافی نازک لایهSunflower - گل آفتابگردانglycerol 3-phosphate - گلیسرول 3-فسفات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The glycerol 3-phosphate acyltransferase (GPAT, EC 2.3.1.15) from sunflower (Helianthus annuus L.) microsomes has been characterised and partially purified. The in vitro determination of activity was optimized, and the maximum value for GPAT activity identified between 15 and 20 days after flowering. The apparent Michaelis-Menten Km for the glycerol 3-phosphate was 354 μM. The preferred substrates were palmitoyl-CoA = linoleoyl-CoA > oleoyl-CoA with the lowest activity using stearoyl-CoA. High solubilisation was achieved using 0.75% Tween80 and the solubilised GPAT was partially purified by ion-exchange chromatography using a Hi-Trap DEAE FF column, followed by gel filtration chromatography using a Superose 12 HR column. The fraction containing the GPAT activity was analysed by SDS-PAGE and contained a major band of 60.1 kDa. Finally, evidence is provided which shows the role of GPAT in the asymmetrical distribution, between positions sn-1 and sn-3, of saturated fatty acids in highly saturated sunflower triacylglycerols. This work provides background information on the sunflower endoplasmic reticulum GPAT which may prove valuable for future modification of oil deposition in this important crop.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 48, Issues 2â3, FebruaryâMarch 2010, Pages 73-80
Journal: Plant Physiology and Biochemistry - Volume 48, Issues 2â3, FebruaryâMarch 2010, Pages 73-80
نویسندگان
Noemà Ruiz-López, Rafael Garcés, John L. Harwood, Enrique MartÃnez-Force,