کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2015477 | 1541975 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
HPCE quantification of 5-methyl-2â²-deoxycytidine in genomic DNA: Methodological optimization for chestnut and other woody species
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
5-methyl-2′-deoxycytidineHPCECastanea sativaCytidine5mdCCTAB2′-deoxyadenosine - 2'-deoxyadenosine2′-deoxycytidine - 2'-deoxycytidine2′-deoxyguanosine - 2'-deoxyguanosineDNA - DNA یا اسید دزوکسی ریبونوکلئیکAdenosine - آدنوزینUridine - ادرینdeoxyribonucleic acid - اسید deoxyribonucleicRNA - اسید ریبونوکلئیکribonucleic acid - اسید ریبونوکلئیکHigh performance capillary electrophoresis - الکتروفورز مویرگی با کارایی بالاEpigenetic - اپی ژنتیکThymidine - تیمیدینdeoxythymidine - دگزوسیتیمیدینHexadecyltrimethyl ammonium bromide - هگزادیدیل تریمیل آمونیوم برومیدhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراguanosine - گوانوزین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Quantification of deoxynucleosides using micellar high-performance capillary electrophoresis (HPCE) is an efficient, fast and inexpensive evaluation method of genomic DNA methylation. This approach has been demonstrated to be more sensitive and specific than other methods for the quantification of DNA methylation content. However, effective detection and quantification of 5-methyl-2â²-deoxycytidine depend of the sample characteristics. Previous works have revealed that in most woody species, the quality and quantity of RNA-free DNA extracted that is suitable for analysis by means of HPCE varies among species of the same gender, among tissues taken from the same tree, and vary in the same tissue depending on the different seasons of the year. The aim of this work is to establish a quantification method of genomic DNA methylation that lends itself to use in different Castanea sativa Mill. materials, and in other angiosperm and gymnosperm woody species. Using a DNA extraction kit based in silica membrane has increased the resolutive capacity of the method. Under these conditions, it can be analyzed different organs or tissues of angiosperms and gymnosperms, regardless of their state of development. We emphasized the importance of samples free of nucleosides, although, in the contrary case, the method ensures the effective separation of deoxynucleosides and identification of 5-methyl-2â²-deoxycytidine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 46, Issues 8â9, AugustâSeptember 2008, Pages 815-822
Journal: Plant Physiology and Biochemistry - Volume 46, Issues 8â9, AugustâSeptember 2008, Pages 815-822
نویسندگان
Rodrigo Hasbún, LuÃs Valledor, José L. RodrÃguez, Estrella Santamaria, Darcy RÃos, Manuel Sanchez, MarÃa J. Cañal, Roberto RodrÃguez,