کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2016401 1067656 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves
چکیده انگلیسی

Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.

Research highlights
► LPPs catalyse the dephosphorylation of DGPP and PA.
► Atlpp2-2 knockout mutant showed a higher PA level and PAK activity than Col-0.
► Inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0.
► Atlpp2-2 and Col-0 plants accumulated ABA to the same extent.
► Atlpp2-2 is an ABA-signalling mutant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 49, Issue 3, March 2011, Pages 357–362
نویسندگان
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