کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2016443 1541972 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
SDS-dependent proteases induced by ABA and its relation to Rubisco and Rubisco activase contents in rice leaves
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
SDS-dependent proteases induced by ABA and its relation to Rubisco and Rubisco activase contents in rice leaves
چکیده انگلیسی

Protease activities and its relation to the contents of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase were investigated in detached leaves of rice (Oryza sativa L.) floated on the solutions containing abscisic acid (ABA) or benzyladenine (BA). Rubisco and Rubisco activase contents were decreased during the time course and the decreases were enhanced by ABA and suppressed by BA. The decrease in Rubisco activase was faster than that in Rubisco. SDS-dependent protease activities at 50–70 kDa (rice SDS-dependent protease: RSP) analyzed by the gelatin containing PAGE were significantly enhanced by ABA. RSPs were also increased in attached leaves during senescence. RSPs had the pH optimum of 5.5, suggesting that RSPs are vacuolar protease. Both decrease in Rubisco and Rubisco activase contents and increase in RSPs activities were suppressed by cycloheximide. These findings indicate that the activities of RSPs are well correlated with the decrease in these protein contents. Immunoblotting analysis showed that Rubisco in the leaf extracts was completely degraded by 5 h at pH 5.5 with SDS where it was optimal condition for RSPs. However, the degradation of Rubisco did not proceed at pH 7.5 without SDS where it is near physiological condition for stromal proteins. Rubisco activase was degraded at similar rate under both conditions. These results suggest that RSPs can functions in a senescence related degradation system of chloroplast protein in rice leaves. Rubisco activase would be more susceptible to proteolysis than Rubisco under physiological condition and this could affect the contents of these proteins in leaves.

Research highlights
► Decrease in Rubisco and Rubisco activase contents were enhanced by ABA.
► SDS-dependent protease activities were enhanced by ABA and senescence.
► Rubisco and Rubisco activase can be degraded by SDS-dependent protease.
► Rubisco activase would be more susceptible to proteolysis than Rubisco.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 48, Issues 10–11, October–November 2010, Pages 808–812
نویسندگان
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