Partial characterization and expression of leaf catalase in the CAM-inducible halophyte Mesembryanthemum crystallinum L.

Catalase (CAT; EC 1.11.1.6) isolated from leaves of the halophytic plant Mesembryanthemum crystallinum is characterized by a high apparent molecular mass of about 320 kDa, and high resistance to denaturing agents (10% ME). SDS-treatment breaks active oligomeric CAT into the less active and putatively dimeric form of 160 kDa apparent molecular mass. Three subunits are resolved after denaturing PAGE: 79, 74 and 62 kDa. Higher molecular masses of subunits coincide with increased activity of CAT. M. crystallinum leaf CAT reveals a diel variation in the resistance to denaturing factors and the stability of CAT is increased in a light-dependent manner both in C3- and in CAM-induced plants. Unchanged level of leaf CAT transcripts is documented in the diurnal cycle of C3 plants and after salinity-induced crassulacean acid metabolism (CAM).