کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2019769 | 1542224 | 2012 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Analysis of sphingolipid and prostaglandin synthesis during zymosan-induced inflammation Analysis of sphingolipid and prostaglandin synthesis during zymosan-induced inflammation](/preview/png/2019769.png)
Sphingosine-1-phosphate (S1P) is generated through phosphorylation of sphingosine by two sphingosine kinases (SPHK-1 and -2). As extra- and intracellular messenger S1P fulfils multiple roles in inflammation such as mediating proinflammatory inputs or acting as chemoattractant. In addition, S1P induces cyclooxygenase-2 (COX-2) expression and the synthesis of proinflammatory prostanoids in several cell types. Here, we analysed in vivo the regulation of S1P level as well as potential interactions between S1P and COX-dependent prostaglandin synthesis during zymosan-induced inflammation. S1P and prostanoid levels were determined in the blood and at the site of inflammation under basal conditions and during zymosan-induced inflammation using wild type and SPHK-1 and -2 knockout mice. We found that alterations in S1P levels did not correlate with changes in plasma- or tissue-concentrations of the prostanoids as well as COX-2 expression. In the inflamed tissue S1P and prostanoid concentrations were reciprocally regulated. Prostaglandin levels increased over 6 h, while S1P and sphingosine level decreased during the same time, which makes an induction of prostanoid synthesis by S1P in zymosan-induced inflammation unlikely. Additionally, despite altered S1P levels wild type and SPHK knockout mice showed similar behavioural nociceptive responses and oedema sizes suggesting minor functions of S1P in this inflammatory model.
► Prostaglandin and S1P levels in blood as well as inflamed tissue were determined.
► Wild type and SPHK knockout mice were tested for zymosan-induced inflammation.
► Changes in S1P and prostaglandin levels in the blood were not correlated.
► In inflamed tissue S1P and prostaglandins were reciprocally regulated.
► Oedema size and nociceptive behaviour were similar in wild type and SPHK knockout mice.
Journal: Prostaglandins & Other Lipid Mediators - Volume 99, Issues 1–2, October 2012, Pages 15–23