کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2019962 | 1542242 | 2009 | 6 صفحه PDF | دانلود رایگان |
In this paper we investigated the possible involvement of prostaglandin E synthases (PGESs) in compensatory mechanism. Our findings showed that microsomal (m)PGES-1 expression was significantly up-regulated in COX knock-out (K/O) cells whereas the expression of cytosolic PGES was not changed indicating that the induction of mPGES-1 may, at least in part, contribute to the substantial increase of PGE2 production in COX K/O cell lines. The selective up-regulation of mPGES-1 in COX-2 K/O cells suggests that mPGES-1 may be metabolically coupled with COX-1 for PGE2 formation. Addition of arachidonic acid caused significant induction of mPGES-1 and COX-2 in WT cells, whereas COX-1 and cPGES were not affected. Our earlier and the current studies demonstrate the coregulation of cPLA2, COX, and mPGES-1, in PGE2 synthesis pathway, and that these enzymes contribute to the elevation of PGE2 level when one COX isoform is absent.
Journal: Prostaglandins & Other Lipid Mediators - Volume 88, Issues 3–4, April 2009, Pages 111–116