کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2021491 1542352 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gram scale separation of casein proteins from whole casein on a Source 30Q anion-exchange resin column utilizing fast protein liquid chromatography (FPLC)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Gram scale separation of casein proteins from whole casein on a Source 30Q anion-exchange resin column utilizing fast protein liquid chromatography (FPLC)
چکیده انگلیسی

Casein is used as an additive in binders or paints and as such exhibits unique properties which might be based on the properties of certain subproteins in the complex whole casein mixture. Therefore, the separation of whole casein (CN) from cow milk was performed on a gram scale in order to yield sufficient amounts of the protein subfractions α-, β-, and κ-casein for further testing utilizing fast protein liquid chromatography (FPLC) and preceding enrichment in the case of κ-casein. Construction chemical grade casein, which differs in quality from dairy grade casein, was used for separation because of our interest in the proteins responsible for plastification of cementitious systems such as mortar. The solubilized proteins were separated chromatographically via ion exchange chromatography (IEX) and the subsequently desalted protein fractions were tested for purity by isoelectric focusing (IEF).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 60, Issue 2, August 2008, Pages 176–181
نویسندگان
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