کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2022301 | 1542363 | 2016 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
β-Galactoside-mediated tissue organization during islet reconstitution
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کلمات کلیدی
RCAβ-GalactosideRicinus communis agglutininUEAConAWGAMAASSAConcanavalin AFITCECADMEMMaackia amurensis agglutinin - Maackia amurensis آگلوتیینینDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccoulex europaeus agglutinin - آلکس یوروپس آگلوتینینLCA - ارزیابی چرخه حیاتSugar chain - زنجیر شکرfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتLectin - لکتینMethylcellulose - متیل سلولزWheat germ agglutinin - گیاه گندم آگلوتیینین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have previously reported that multi-cellular heteroaggregates comprising murine pancreatic α (αTC1.6) and β (MIN6-m9) cell lines spontaneously acquired islet-like architecture and displayed higher insulin secretion rates. However, the mechanisms of self-organization remain unclear. The objective of this study is to examine the possibility that a sugar chain participates in the mutual recognition of the cells during reconstitution of the islet-like structure in vitro. Using a lectin-binding assay, we identified Erythrina cristagalli agglutinin (ECA), which particularly recognizes the β-galactoside structure on the surfaces of MIN6-m9 cells. The self-organization of αTC1.6 and MIN6-m9 was obstructed using ECA-bound MIN6-m9 cells. Lactose neutralized the ECA's inhibitory effect on the autonomous rearrangement of αTC1.6 and MIN6-m9 cells, indicating that the inhibition of cell arrangement by ECA was mediated via β-galactoside. We concluded that a β-galactoside sugar chain was central to the reconstitution of the pancreatic islet-like architecture in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regenerative Therapy - Volume 3, March 2016, Pages 11-14
Journal: Regenerative Therapy - Volume 3, March 2016, Pages 11-14
نویسندگان
Sae Kamitori, Yasuhiro Ozeki, Nobuhiko Kojima,