کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2032311 1542872 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Frankincense derived heavy terpene cocktail boosting breast cancer cell (MDA-MB-231) death in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Frankincense derived heavy terpene cocktail boosting breast cancer cell (MDA-MB-231) death in vitro
چکیده انگلیسی

ObjectiveTo investigate the anti-cancer effect of frankincense derived heavy oil obtained by Soxhlet extraction method on breast cancer cells (MDA-MB-231), and to study its chemical profile using gas chromatography mass spectrometry analysis.MethodsHexane was used to extract heavy oil from frankincense resin. Chemical profiling of heavy oil was done using Perkin Elmer Clarus GC system with mass spectrometer. MDA-MB-231 cells were treated with different dilutions (1:1 000, 1:1 500, 1:1 750, 1:2 000, 1:2 250, 1:2 500, 1:2 750, 1:3 000, 1:3 250) of heavy oil for 24 h. The cells were observed by using light microscopy. Cell viability was measured by MTT assay.ResultsGas chromatography mass spectrometry chemical profiling of frankincense derived heavy oil revealed the presence of terpenes such as α-pinene (61.56%), α-amyrin (20.6%), β-amyrin (8.1%), β-phellandrene (1.47%) and camphene (1.04%). Heavy terpene cocktail induced significant MDA-MB-231 cell death at each concentration tested. Noticeably, very low concentration of Soxhlet derived heavy terpenes elicits considerable cytotoxicity on MDA-MB-231 cells compared to hydro distillated essential oil derived from frankincense resin.ConclusionsExtracting anti-cancer active principle cocktail by simple Soxhlet method is cost effective and less time consuming. Our in vitro anti-cancer data forms the rationale for us to test heavy terpene complex in breast cancer xenograft model in vivo. Furthermore, fractionation and developing frankincense heavy terpene based breast cancer drug is the major goal of our laboratory.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Pacific Journal of Tropical Biomedicine - Volume 5, Issue 10, October 2015, Pages 824–828
نویسندگان
, , ,