کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2032721 1542876 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Morphology and viability of adult Fasciola gigantica (giant liver flukes) from Philippine carabaos (Bubalus bubalis) upon in vitro exposure to lead
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
Morphology and viability of adult Fasciola gigantica (giant liver flukes) from Philippine carabaos (Bubalus bubalis) upon in vitro exposure to lead
چکیده انگلیسی

ObjectiveTo evaluate the effects of lead in the morphology and viability of Fasciola gigantica (F. gigantica) (giant liver fluke) isolated from infected livers of carabaos in vitro using the following concentrations of lead: 0, 100, 150 and 200 mg/L.MethodsIn vitro viability and motility assay was conducted to evaluate the effects of lead using 1% methylene blue as the vital dye for assessment of the flukes' viability.ResultsResults indicate that F. gigantica can tolerate lead exposure as high as 200 mg/L with visible morphological variations. Upon exposure to lead, liver flukes tend to curl and excrete black precipitates as a sign of physiological stress response. Furthermore, the lethal concentration (LC50) of lead against F. gigantica in vitro was 160 mg/L.ConclusionsIn conclusion, tolerance of liver flukes to high levels of lead suggests its potential as a possible biomarker of environmental pollution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Pacific Journal of Tropical Biomedicine - Volume 5, Issue 6, June 2015, Pages 493–496
نویسندگان
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