کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2034755 | 1543053 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The potential of circulating extracellular small RNAs (smexRNA) in veterinary diagnostics-Identifying biomarker signatures by multivariate data analysis
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کلمات کلیدی
reads per millionPLSRPMexRNAextracellular RNApiRNAOPLSSmall RNA-seqPCA - PCAEuropean Union - اتحادیه اروپاcon - باDiscriminant analysis - تجزیه و تحلیل دائمیMultivariate data analysis - تجزیه و تحلیل داده های چند متغیرهPrincipal component analysis - تحلیل مولفههای اصلی یا PCATranscriptomics - متن ترانهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAcontrol group - گروه کنترل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
After running the data evaluation pipeline, the proportion of miRNAs (microRNAs) and piRNAs (PIWI-interacting small non-coding RNAs) on the total sequenced reads was calculated. Additionally, top 10 signatures were compared which revealed that the readcount data sets were highly affected by the most abundant miRNA and piRNA profiles. To evaluate the discriminative power of multivariate data analyses to identify animals after veterinary drug application on the basis of smexRNAs, OPLS-DA was performed. In summary, the quality of miRNA models using all mapped reads for both treatment groups (animals treated with steroid hormones or the β-agonist clenbuterol) is predominant to those generated with combined data sets or piRNAs alone. Using multivariate projection methodologies like OPLS-DA have proven the best potential to generate discriminative miRNA models, supported by small RNA-Seq data. Based on the presented comparative OPLS-DA, miRNAs are the favorable smexRNA biomarker candidates in the research field of veterinary drug abuse.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomolecular Detection and Quantification - Volume 5, September 2015, Pages 15-22
Journal: Biomolecular Detection and Quantification - Volume 5, September 2015, Pages 15-22
نویسندگان
Spornraft Melanie, Kirchner Benedikt, Michael W. Pfaffl, Riedmaier Irmgard,