کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2051773 1074209 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture
چکیده انگلیسی

Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfect Arabidopsis cells. Zeocin-resistant Arabidopsis calli were used to generate a suspension cell culture. Detection of viral proteins and RNAs after 18 months in culture demonstrated persistent replication of the replicon. The Arabidopsis cell culture yielded soluble, active replication complexes, providing a useful tool to study host factors involved in TYMV replication.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 2, 23 January 2007, Pages 337–341
نویسندگان
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