کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2058264 1543944 2010 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات علوم زمین و سیاره ای (عمومی)
پیش نمایش صفحه اول مقاله
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia
چکیده انگلیسی

Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O2 L− 1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes involved in the response to hypoxia in comparison to normoxic conditions. The hypoxic response was maximal at day 20. The principal biological processes up-regulated by hypoxic stress were antioxidant defense and the respiratory chain compartment, suggesting oxidative stress caused by hypoxia or an anticipatory response for normoxic recovery. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C. gigas.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Genomics - Volume 3, Issues 3–4, September–December 2010, Pages 133–143
نویسندگان
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