کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2058389 | 1076226 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Three embryonic myosin heavy chain genes encoding different motor domain structures from common carp show distinct expression patterns in cranial muscles Three embryonic myosin heavy chain genes encoding different motor domain structures from common carp show distinct expression patterns in cranial muscles](/preview/png/2058389.png)
Three embryonic myosin heavy chain (MYH) genes >> (MYHs) including MYHemb1, MYHemb2 and MYHemb3 and encoding a C-terminal part of MYH were previously cloned and demonstrated to be expressed transiently in this order during development of common carp Cyprinus carpio embryos. The present study determined the full-length cDNA nucleotide sequences encoding the motor domain of the three MYHs, suggesting the implication of loop 1 and loop 2 sequences for the differences in the motor functions. Phylogenetic analysis based on the full-length amino acid sequences showed that MYHemb1 and MYHemb2 both belong to the fast types, though clearly differ from fast-type MYHs expressed in adult fast muscle previously reported. In contrast, MYHemb3 was in a clade containing slow/cardiac type. Whole-mount immunostaining and in situ hybridization showed that the transcripts of the three embryonic MYHs are localized in the same or different cranial muscles of common carp larvae, suggesting that the three MYHs function cooperatively or individually in various cranial muscles.
Journal: Marine Genomics - Volume 3, Issue 1, March 2010, Pages 1–9