کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067881 | 1077915 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of C-terminus of Kir7.1 potassium channel in cell-surface expression
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کلمات کلیدی
RIPAKirPDZMDCKPSD-95/DLG/ZO-1endoplasmic reticulum - شبکه آندوپلاسمی Subcellular localization - محلی سازی سلولMutagenesis - موتاژنزhemagglutinin - هماگلوتینینTransmembrane protein - پروتئین ترانزیتیcasein kinase - کازئین کینازIon channel - کانال یونیMadin-Darby canine kidney - کلیه های سگ کوچولو Madin-Darby
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Inward rectifier K+ channel Kir7.1 is predominantly expressed on the plasma membrane of a variety of ion-transporting epithelia. The electrophysiological property of Kir7.1 has been well characterized but the mechanism underlying the plasma-membrane targeting remains elusive. To address this issue, we examined the effect of deletion and site-directed mutagenesis on the plasma-membrane localization of Kir7.1 in Madin-Darby canine kidney cells by immunofluorescence microscopy and cell-surface biotinylation. Although deletions of up to 37 amino acid residues from the C-terminus had no effect, further deletion resulted in accumulation of the mutant proteins in intracellular membranes. No sequence motif for subcellular targeting was found in the distal C-terminal region. The cell-surface expression of the deletion mutant lacking 38 or 40 C-terminal residues was restored by addition of one or three alanine residues, respectively, to the C-terminus end. These results suggest that the C-terminal length plays an important role in the plasma-membrane localization of Kir7.1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 30, Issue 3, March 2006, Pages 270-277
Journal: Cell Biology International - Volume 30, Issue 3, March 2006, Pages 270-277
نویسندگان
Toru Tateno, Nobuhiro Nakamura, Yukio Hirata, Shigehisa Hirose,