کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2067971 1077922 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinectin participates in microtubule-dependent hormone secretion in pancreatic islet β-cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوفیزیک
پیش نمایش صفحه اول مقاله
Kinectin participates in microtubule-dependent hormone secretion in pancreatic islet β-cells
چکیده انگلیسی
Kinectin (KNT) is a candidate membrane receptor for kinesin in the movement of intracellular organelles along microtubules. Isoforms of KNT exist containing different combinations of six small (residues 23-33) variable domains (vd) vd1-6 within the C-terminus. Here we investigate a role for KNT and its isoform KNTvd4− in the transport of amylin and insulin-containing secretory vesicles in the pancreatic islet β-cell line RINm5F. KNTvd4− lacks vd4 that forms the kinesin-binding domain, and hence its role in the cell is an enigma. We report that amylin-containing vesicles also contained insulin, and exhibited microtubule, and small G-protein-dependent secretion. Knockdown of KNT by small interference RNA (siRNA) inhibited amylin expression and secretion. In contrast, recombinant KNTvd4− overexpressed in RINm5F cells associated with amylin-containing vesicles and inhibited amylin secretion, but had no discernible affect on amylin expression. The data suggests that both KNT and KNTvd4− participate in microtubule-dependent secretion of amylin in islet β-cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 30, Issue 11, November 2006, Pages 885-894
نویسندگان
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