کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2076857 1545003 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of the oscillatory kinetics of glycolytic intermediates in a yeast extract by FT-IR spectroscopy
موضوعات مرتبط
مهندسی و علوم پایه ریاضیات مدل‌سازی و شبیه سازی
پیش نمایش صفحه اول مقاله
Analysis of the oscillatory kinetics of glycolytic intermediates in a yeast extract by FT-IR spectroscopy
چکیده انگلیسی

In the present work we demonstrate that FT-IR spectroscopy is a powerful tool for the time resolved and noninvasive measurement of multi-substrate/product interactions in complex metabolic networks as exemplified by the oscillating glycolysis in yeast extract. We found that many of the glycolytic intermediates can be identified with FT-IR spectroscopy. For this, we have constructed a spectral library of most of the glycolytic intermediates and obtained the kinetics of single components in spectra from glycolysing yeast extract by the use of mathematical fitting procedures. The results are in good agreement with the known phase relationships of oscillatory glycolysis. They provide the basis for future application of this method to investigate the energy metabolism of living cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosystems - Volume 83, Issues 2–3, February–March 2006, Pages 188–194
نویسندگان
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