کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2083975 1545340 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bufalin loaded biotinylated chitosan nanoparticles: An efficient drug delivery system for targeted chemotherapy against breast carcinoma
ترجمه فارسی عنوان
بوفالین نانو ذرات کیتوزان بیوتینیل را بارگذاری می کند: یک سیستم تحویل دارویی مناسب برای شیمی درمانی هدفمند در برابر کارسینوم پستان
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• We prepared biotinylated chitosan nanoparticles encapsulating bufalin (Bu-BCS-NPs).
• Bu-BCS-NPs show improved water solubility, and slow release rate of bufalin.
• Superior intracellular uptake is obtained due to biotinylation.
• Much stronger cytotoxicity, ROS and apoptosis induction than free bufalin.
• Bu-BCS-NPs show markedly improved in vivo therapeutic effects.

Bufalin is a traditional oriental medicine which is known to induce apoptosis in many tumor cells, and it is thus considered as a new anticancer therapeutic. By now, most of the studies of bufalin are in vitro, however in vivo evaluations of its therapeutic efficacy are less and are in great demand for its development toward anticancer drug. One of the problems probably hampering the development of bufalin is the lack of tumor selectivity, which may reduce the therapeutic effect as well as showing side effects. To overcome this drawback, in this study, we designed a tumor-targeted drug delivery system of bufalin based on enhanced permeability and retention (EPR) effect, by using biotinylated chitosan, resulting in bufalin encapsulating nanoparticles (Bu-BCS-NPs) with mean hydrodynamic size of 171.6 nm, as evidenced by dynamic light scattering and transmission electron microscope. Bu-BCS-NPs showed a relative slow and almost linear release of bufalin, and about 36.8% of bufalin was released in 24 h when dissolved in sodium phosphate buffer. Compared to native bufalin, Bu-BCS-NPs exhibited a stronger cytotoxicity against breast cancer MCF-7 cells (IC50 of 0.582 μg/ml vs 1.896 μg/ml of native bufalin). Similar results were also obtained in intracellular reactive oxygen species production, apoptosis induction, and decrease in mitochondria membrane potential. These results may contribute to the rapid intracellular uptake of nanoparticles, partly benefiting from the highly expressed biotin receptors in tumor cells. In vivo studies using MCF-7 tumor models in nude mice confirmed the remarkable therapeutic effect of Bu-BCS-NPs. These findings suggest the potential of Bu-BCS-NPs as an anticancer drug with tumor targeting property.

Figure optionsDownload high-quality image (82 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 87, Issue 3, August 2014, Pages 445–453
نویسندگان
, , , , , , , , , , ,