کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2089232 | 1545785 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Soluble human leukocyte antigen: A diagnostic indicator of rheumatoid arthritis?
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
mAbRheumatoid arthritis - آرتریتروماتوئیدMonoclonal antibody - آنتی بادی مونوکلونالHLA - آنتیژن گلبول سفید انسانیhealthy donor - اهدا کننده سالمELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSerum - سرمrheumatoid factor - عامل روماتوئیدSoluble - قابل حل استsynovial fluid - مایع زلالهای، مایع مفصلی، مایع سینوویالMolecular weight - وزن مولکولیClass II - کلاس دوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Soluble human leukocyte antigen: A diagnostic indicator of rheumatoid arthritis? Soluble human leukocyte antigen: A diagnostic indicator of rheumatoid arthritis?](/preview/png/2089232.png)
چکیده انگلیسی
Early stage rheumatoid arthritis (RA) is often difficult to diagnose because initial symptoms are non-specific. To aid diagnosis, suitable serological diagnostic markers are sought. Elevated levels of soluble MHC class II (soluble human leukocyte antigen; sHLA-DR) in human serum have been associated with rheumatoid and 'rheumatoid-like' autoimmune diseases. As a result, sHLA-DR has been suggested as a specific marker of RA. However, reported levels of sHLA-DR in sera of healthy donors vary significantly and the mechanism of release of HLA-DR into serum is poorly understood. Investigations into the diagnostic potential of this molecule necessitate the development of a sensitive and specific sHLA-DR assay. We have investigated multiple ELISA setups to develop such an assay and false positive signal has been carefully removed using a combination of isotype-matched controls and immuno-depletion. sHLA-DR levels in sera of RA patients were not significantly different from those in healthy donors which suggests sHLA-DR has limited utility in the diagnosis of RA. In RA patients, we detected high levels of sHLA-DR in aspirated synovial fluid (SF), but this did not correlate with sHLA-DR levels in serum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 315, Issues 1â2, 31 August 2006, Pages 19-26
Journal: Journal of Immunological Methods - Volume 315, Issues 1â2, 31 August 2006, Pages 19-26
نویسندگان
M.W. Hayman, M.T.A. van Beijnen, L.K. Stamp, M.B. Spellerberg, J.L. O'Donnell,