Application of an ELISA-type screen printed electrode-based potentiometric assay to the detection of Cryptosporidium parvum oocysts

• We developed a functionalisation protocol for C. parvum oocysts capture.
• We optimised a specific sandwich-type potentiometric detection of C. parvum.
• 5 × 102C. parvum oocysts per mL were detected in 60 minutes.

We report a novel electrochemical method for the rapid detection of the parasitic protozoan, Cryptosporidium parvum. An antibody-based capture format was transferred onto screen-printed electrodes and the presence of horseradish peroxidase-labelled antibodies binding to the oocysts was potentiometrically detected. This method allowed the detection of 5 × 102Cryptosporidium oocysts per mL in 60 min.